Pyrrolidone is a reusable cell storage box. For most animal cells, it can save a lot of time for repeated cell transfers. Reducing -1 to -3℃ per minute can make cells better adapt to the frozen state. At present, the commonly used method in most laboratories is the gradient cooling method: place it at 4℃ for 30 minutes, then transfer it to -20℃ for 2 hours, then transfer it to a -80℃ refrigerator for freezing overnight, and transfer the cryopreserved tube to a liquid nitrogen tank for long-term storage the next day. Put the prepared cell cryopreserved tube into the cryopreservation box and transfer it to the liquid nitrogen tank directly after overnight at -80℃. No matter which freezing method is used, it is important to be quick in the process of transferring the storage location. It is recommended to keep the cryopreserved tube in dry ice to keep the temperature constant during the transfer process to avoid the impact of temperature changes on the cells during the transfer process.
For continuous low-temperature storage, the cell temperature should always be kept below -130℃ to achieve the best survival rate. If the storage temperature is not well controlled, the survival rate will decrease over time. It is recommended to store cells in liquid nitrogen for a long time. It is necessary to manually check the airtightness of the liquid nitrogen tank and whether the amount of liquid nitrogen is sufficient regularly to avoid damage to cells due to storage conditions. Commonly used drugs for surgical dressing changes, also known as changing dressings, include checking the wound, removing pus and secretions, cleaning the wound and covering the dressing. It is an important surgical operation to prevent and control wound infection, factors that hinder wound healing, and promote wound healing.
(Pyrrolidone) Most patients who have undergone surgery need to undergo several dressing changes before the surgical incision heals. Various different drugs are often used in the dressing process. Let's take a look at the characteristics and functions of some commonly used drugs.
1. Iodine tincture and aner iodine. Iodine tincture is an amorphous combination of elemental iodine and polyvinyl pyrrolidone (Povidone). Polyvinyl pyrrolidone can dissolve and disperse 9%~12% of iodine, and it appears as a purple-black liquid. However, medical iodine tincture is usually in a lower concentration (1% or less) and appears light brown. Iodine tincture has a broad spectrum of effects and can kill propagules, spores, protozoa and some viruses. (Pyrrolidone) It is mainly used for skin, has little irritation to mucous membranes, does not require ethanol deiodination, has low toxicity, is non-corrosive to objects, and the color is easy to wash off. Iodine tincture is superior to iodine tincture in terms of its application range (mucous membranes, skin, etc.) and less reaction.
